PI: Tanja Kuhlmann
INSTITUTE Westfälische Wilhelms-Universit-ät Münster
ADDRESS Schlossplatz 2, 48149 Münster
The Kuhlmann lab has a strong background in MS neuropathology and oligodendroglial biology. It has published several studies analyzing the histopathology of MS lesions with a special focus on oligodendroglial as well as axonal damage and repair mechanisms.19,122,125-127 They established markers to identify different oligodendroglial populations in developmental and in adult human tissue samples including early and chronic MS lesions.19,128 To be able to understand the mechanisms underlying the pathological characteristics of MS lesions and to address functional questions in oligodendroglial biology, the Kuhlmann lab established the isolation and culturing of primary murine oligodendrocytes and assays to study the proliferation, migration, differentiation and survival of these cells as well as cerebellar slice cultures.61,159,160 Using these assays they dissected cell death mechanisms in primary oligodendrocytes and identified indomethacin as a compound that promotes oligodendroglial differentiation as well as remyelination, at least partly by inhibiting the Wnt/b-catenin signalling pathway.61 In addition, the Kuhlmann lab analyzes pathways modulating oligodendroglial differentiation in primary murine oligodendrocytes and murine animal models. In order to translate their findings back from murine to human cells the Kuhlmann lab established a fast, reliable and reproducible protocol to generate oligodendrocytes from hiPSC as well as protocols to derive neurons and astrocytes from hiPSC.161,162
Pertinent to the proposal, Partner 8 will be responsible of implementing in vivo and in vitro models of demyelination (murine and human) as well as of assessing pathological validation in human preserved pathological samples.
Human iPSC-derived Oligos (iOLS)
- Khulmann, Uni Muenster